PXD026825

PXD026825 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes of ZIKV strains and DENV in neural stem cells
Description	Brain abnormalities and congenital malformations have been linked to the circulating strain of Zika virus (ZIKV) in Brazil since 2016 during the microcephaly outbreak; however, the molecular mechanisms behind several of these alterations and differential viral molecular targets have not been fully elucidated. Here we explore the proteomic alterations induced by ZIKV by comparing the Brazilian (Br ZIKV) and the African (MR766) viral strains, in addition to comparing them to the molecular responses to the Dengue virus (DENV). Neural stem cells (NSCs) derived from induced pluripotent stem (iPSCs) were cultured both as monolayers and in suspension (resulting in neurospheres), which were then infected with ZIKV (Br ZIKV or ZIKV MR766) or DENV to assess alterations within neural cells. Large-scale proteomic analyses allowed the comparison not only between viral strains but also regarding the two- and three-dimensional cellular models of neural cells derived from iPSCs, and the effects on their interaction. Altered pathways and biological processes were observed, related to cell death, cell cycle dysregulation, and neurogenesis. These results reinforce already published data and provide further information regarding the biological alterations induced by ZIKV and DENV.
HostingRepository	PRIDE
AnnounceDate	2022-06-29
AnnouncementXML	Submission_2022-06-29_12:37:47.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliana Minardi Nascimento
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-21 03:03:30	ID requested
⏵ 1	2022-06-29 12:37:48	announced

Nascimento JM, Gouv, ê, a-Junqueira D, Zuccoli GS, Pedrosa CDSG, Brand, ã, o-Teles C, Crunfli F, Antunes ASLM, Cassoli JS, Karmirian K, Salerno JA, de Souza GF, Muraro SP, Proenca-M, ó, dena JL, Higa LM, Tanuri A, Garcez PP, Rehen SK, Martins-de-Souza D, Zika Virus Strains and Dengue Virus Induce Distinct Proteomic Changes in Neural Stem Cells and Neurospheres. Mol Neurobiol, 59(9):5549-5563(2022) [pubmed]

submitter keyword: neurodevelopment, microcephaly, Flaviviridae, NSC, iPSC, zika, dengue

Daniel Martins-de-Souza
contact affiliation	Lab of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
contact email	dmsouza@unicamp.br
lab head
Juliana Minardi Nascimento
contact affiliation	University of Campinas
contact email	juminardi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD026825

PRIDE project URI

• PRIDE
  1. PXD026825
     1. Label: PRIDE project
     2. Name: Proteomic changes of ZIKV strains and DENV in neural stem cells