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PXD026822

PXD026822 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis
DescriptionHepatocellular carcinoma (HCC) is frequently characterized by metabolic and immune remodeling in the tumor microenvironment. We previously discovered that liver-specific deletion of fructose-1, 6-bisphphotase 1 (FBP1), a gluconeogenic enzyme ubiquitously suppressed in HCC tissues, promotes liver tumorigenesis, and induces metabolic and immune perturbations closely resembling human HCC. However, the underlying mechanisms remain incompletely understood. Here we reported that FBP1-deficient livers exhibit diminished amounts of natural killer (NK) cells and accelerated tumorigenesis. Using the diethylnitrosamine-induced HCC mouse model, we analyzed potential changes in the immune cell populations purified from control and FBP1-depleted livers and found that NK cells were strongly suppressed. Mechanistically, FBP1 attenuation in hepatocytes derepresses an EZH2-dependent transcriptional program to inhibit PKLR expression. This leads to reduced levels of PKLR cargo proteins sorted into hepatocyte-derived EVs, dampened activity of EV-targeted NK cells, and accelerated liver tumorigenesis. Our study demonstrated that hepatic FBP1 depletion promotes HCC-associated immune remodeling, partly through the transfer of hepatocyte-secreted, PKLR-attenuated EVs to NK cells.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:38:13.504.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterzhijun liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
Instrument6410 Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-20 22:55:31ID requested
12023-03-10 23:15:40announced
22023-11-14 08:38:15announced2023-11-14: Updated project metadata.
Publication List
Liu Z, You Y, Chen Q, Li G, Pan W, Yang Q, Dong J, Wu Y, Bei JX, Pan C, Li F, Li B, Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis. Mol Ther, 30(2):606-620(2022) [pubmed]
Keyword List
submitter keyword: Extracellular vesicle
Contact List
Bo Li
contact affiliationZhongshan School of Medicine Sun Yat-sen University, Guangzhou, China
contact emaillibo47@mail.sysu.edu.cn
lab head
zhijun liu
contact affiliationSun Yat-sen University
contact email13242856926@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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