PXD026804

PXD026804 is an original dataset announced via ProteomeXchange.

Title	Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle
Description	The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Thus, we hypothesised unknown pathways for the uptake of aminophosphonates must exist in seawater. Here, we identify three novel bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose expression is independent of phosphate concentrations (phosphate-insensitive). AepXVW is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphonate binding protein, PhnD, the newly identified AepX is heavily transcribed (~100-fold>PhnD) in the global ocean independently of phosphate and nitrogen concentrations, and has high specificity for 2AEP (S. stellulata AepX Kd 23±4 nM). Collectively, our data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production.
HostingRepository	PRIDE
AnnounceDate	2021-09-07
AnnouncementXML	Submission_2021-09-07_03:50:18.189.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026804
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Pseudomonas putida BIRD-1; NCBI TaxID: 931281; scientific name: Stappia stellulata DSM 5886; NCBI TaxID: 1123294;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-18 06:04:32	ID requested
⏵ 1	2021-09-07 03:50:19	announced

Murphy ARJ, Scanlan DJ, Chen Y, Adams NBP, Cadman WA, Bottrill A, Bending G, Hammond JP, Hitchcock A, Wellington EMH, Lidbury IDEA, Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle. Nat Commun, 12(1):4554(2021) [pubmed]

submitter keyword: Proteomics, LCMSMS, Warwick-RTP, Transporter, phosphorus redox cycle

Dr Ian Lidbury
contact affiliation	BBSRC Discovery Fellow & BMC Microbiome Managing Editor Arthur Willis Environmental Research Centre University of Sheffield Sheffield S10 1AE
contact email	i.lidbury@sheffield.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026804
     1. Label: PRIDE project
     2. Name: Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle