⮝ Full datasets listing

PXD026797

PXD026797 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS_CoV-2 substrate profiling in HEK-293 cell proteomes
DescriptionTo profile the human substrate degradome of the SARS-CoV-2 main viral protease by exposingnative proteome extracts from human embryonic kidney (HEK-293) cells (N = 3) to 3CLpro cleavage. Then, N Terminal Amine Labeling of Substates (TAILS) was used to identify the substrates. Here, heavy [+34] isotope dimethyl labeling is performed at the whole protein level in the 3CLpro-exposed samples, and the P1' side of substrates substrates was revealed by comparing to the samples exposed to inactive mutant labeled with ligh [+28 Da] dimethyl.
HostingRepositoryPRIDE
AnnounceDate2021-11-03
AnnouncementXMLSubmission_2021-11-03_05:29:19.528.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026797
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYoan Machado
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; N-methylmaleimide modified L-cysteine
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-18 03:52:15ID requested
12021-11-03 05:29:19announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: SARS-CoV-2,3CLpro, nsp5, COVID19, Bruker Q-TOF, TAILS-N-terminomics,
Contact List
Christopher M. Overall
contact affiliationCenter for Blood Research, Life Sciences Centre, University of British Columbia; Vancouver, British Columbia, Canada, V6T 1Z3
contact emailchris.overall@ubc.ca
lab head
Yoan Machado
contact affiliationUniversity of British Columbia
contact emailyoan.machado@ubc.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD026797
PRIDE project URI
Repository Record List
[ + ]