PXD026772 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics |
Description | Cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification of C. sinensis leaves. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. Cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method, and subsequently all proteins were subjected to UPLC-MS/MS analysis. A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Among identified CWPs, proteins acting on cell wall polysaccharides, proteases and oxidoreductases represent the top three functional groups. This was the first large-scale investigation of CWPs in C. sinensis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_04:39:19.703.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yanli Liu |
SpeciesList | scientific name: Camellia sinensis; NCBI TaxID: 4442; |
ModificationList | N-glycosylated residue |
Instrument | Orbitrap Fusion; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-18 01:28:24 | ID requested | |
⏵ 1 | 2022-02-17 04:39:20 | announced | |
Publication List
Liu Y, Ma L, Cao D, Gong Z, Fan J, Hu H, Jin X, Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics. BMC Plant Biol, 21(1):384(2021) [pubmed] |
Keyword List
submitter keyword: Camellia sinensis |
leaves |
cell wall proteins; |
Contact List
Yanli Liu |
contact affiliation | Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences |
contact email | zkslyl@16.com |
lab head | |
Yanli Liu |
contact affiliation | Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences |
contact email | zkslyl@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026772
- Label: PRIDE project
- Name: Investigation of cell wall proteins of Camellia sinensis leaves by combining cell wall proteomics and N-glycoproteomics