Added PubMed Id A set of peptides was taken for targeted validation using targeted proteomic approach of Multiple reaction monitoring (MRM)/selected reaction monitoring (SRM). We have performed targeted experiment for two proteins in female gonad tissue of Labeo rohita. Results were matched with a spectral library for the reliability of the data. TSQ Altis Triple Quadrupole Mass spectrometer (Thermo) coupled to an HPLC system was used for acquiring the data. This data represents the significance of PeptideAtlas in targeted experiments.