PXD026748

PXD026748 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro
Description	Here, we applied an ISG15-GlyGly peptidomics approach on HeLa cells producing high levels of ISGylated proteins upon stimulation with IFN-α. After obtaining cellular lysates from wild-type and Isg15-/-cells, the samples were incubated with recombinant wild-type or mutant PLpro to catalyze deISGylation of host cellular proteins. After incubation with PLpro, we continued with trypsin digestion to generate diglycine-modified peptides that were subsequently enriched by immunoprecipitation and identified and quantified by LC-MS/MS. By comparing sites across all four conditions, we uncovered 118 ISGylation sites on 95 proteins as targets of the SARS-CoV-2 protease PLpro.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_07:14:42.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denzel Eggermont
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-16 09:36:03	ID requested
⏵ 1	2021-10-12 07:14:42	announced

Dataset with its publication pending

ProteomeXchange project tag: Covid-19

submitter keyword: ISG15, SARS-CoV-2, interferon

Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
contact email	Francis.Impens@vib-ugent.be
lab head
Denzel Eggermont
contact affiliation	VIB-Ugent Center for Medical Biotechnology
contact email	denzel.eggermont@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD026748

PRIDE project URI

• PRIDE
  1. PXD026748
     1. Label: PRIDE project
     2. Name: Proteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro