PXD026740

PXD026740 is an original dataset announced via ProteomeXchange.

Title	Capturing Salmonella SspH2 Host Targets in Virus-Like Particles
Description	In the context of host-pathogen interactions, gram-negative bacterial virulence factors, such as effectors, may be transferred from bacterial to eukaryotic host cytoplasm by multicomponent Type III protein secretion systems (T3SSs). Central to Salmonella enterica serovar Typhimurium (S. Typhimurium) pathogenesis is the secretion of over 40 effectors by two T3SSs encoded within pathogenicity islands SPI-1 and SPI-2. These effectors manipulate miscellaneous host cellular processes, such as cytoskeleton organization and immune signaling pathways, thereby permitting host colonization and bacterial dissemination. Recent research on effector biology provided mechanistic insights for some effectors. However, for many effectors, clearly defined roles and host target repertoires—further clarifying effector interconnectivity and virulence networks—are yet to be uncovered. Here we demonstrate the utility of the recently described viral-like particle trapping technology Virotrap as an effective approach to catalogue S. Typhimurium effector-host protein complexes (EH-PCs). Mass spectrometry-based Virotrap analysis of the novel E3 ubiquitin ligase SspH2 previously shown to be implicated in modulating actin dynamics and immune signaling, exposed known host interactors PFN1 and -2 and several putative novel, interconnected host targets. Network analysis revealed an actin(-binding) cluster among the significantly enriched hits for SspH2, consistent with the known localization of the S-palmitoylated effector with actin cytoskeleton components in the host. We show that Virotrap complements the current state-of-the-art toolkit to study protein complexes and represents a valuable means to screen for effector host targets in a high-throughput manner, thereby bridging the knowledge gap between effector-host interplay and pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:37:02.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margaux De Meyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-16 04:11:34	ID requested
⏵ 1	2022-02-17 04:37:04	announced

De Meyer M, Fijalkowski I, Jonckheere V, De Sutter D, Eyckerman S, Van Damme P, SspH2 Host Targets in Virus-Like Particles. Front Med (Lausanne), 8():725072(2021) [pubmed]

submitter keyword: SspH2, Salmonella, effector, Virotrap

Petra Van Damme
contact affiliation	Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Belgium
contact email	petra.vandamme@ugent.be
lab head
Margaux De Meyer
contact affiliation	Universiteit Gent, VIB-UGent Center for Medical Biotechnology
contact email	margaux.demeyer@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026740

PRIDE project URI

• PRIDE
  1. PXD026740
     1. Label: PRIDE project
     2. Name: Capturing Salmonella SspH2 Host Targets in Virus-Like Particles