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PXD026733

PXD026733 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Cell Wall Proteome of Craterostigma plantagineum Cell Cultures Habituated to Dichlobenil and Isoxaben
DescriptionThe remarkable desiccation tolerance of the vegetative tissues in the resurrection species Craterostigma plantagineum (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall research has greatly advanced thanks to the use of inhibitors affecting the biosynthesis of e.g. cellulose, since they allowed the identification of the compensatory mechanisms underlying habituation. Considering the innate cell wall plasticity of C. plantagineum, the goal of this investigation was to understand whether habituation to the cellulose biosynthesis inhibitors dichlobenil and isoxaben entailed or not identical mechanisms as known for non-resurrection species and to decipher the cell wall proteome of habituated cells. The results showed that exposure of C. plantagineum calli/cells triggered abnormal phenotypes as reported in non-resurrection species. Additionally, the data demonstrated that it was possible to habituate Craterostigma cells to dichlobenil and isoxaben and that gene expression and proteomics did not follow the same trend. Shotgun and gel-based proteomics revealed a common set of proteins induced upon habituation, but also identified candidates solely induced by habituation to one of the two inhibitors. Finally, it is hypothesized that alterations in auxin levels are responsible for the increased abundance of cell wall-related proteins upon habituation.
HostingRepositoryPRIDE
AnnounceDate2021-11-03
AnnouncementXMLSubmission_2021-11-03_02:38:14.000.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026733
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCéline LECLERCQ
SpeciesList scientific name: Craterostigma plantagineum; NCBI TaxID: 4153;
ModificationListL-kynurenine; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-15 15:44:48ID requested
12021-11-03 02:38:14announced
Publication List
Meyers JM, Ramanathan M, Shanderson RL, Donohue L, Ferguson I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, Wang Y, Khavari PA, The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation. bioRxiv, ():(2021) [pubmed]
Keyword List
submitter keyword: Craterostigma plantagineum
dichlobenil
isoxaben
habituation
cell wall
qPCR
proteomics.
Contact List
Celine LECLERCQ
contact affiliationLIST-ERIN-BEAP, Belvaux, LUXEMBOURG
contact emailceline.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliationLIST-ERIN-ERTP-GreenTech platform
contact emailceline.leclercq@list.lu
dataset submitter
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Dataset FTP location
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