PXD026728

PXD026728 is an original dataset announced via ProteomeXchange.

Title	Rufy4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
Description	We report here that RUFY4, a newly characterized member of the "RUN and FYVE domain-containing" family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its c-terminal FYVE domain. Further, we demonstrate that Rufy4 mRNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_08:59:17.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anshu Bhattacharya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 14:51:58	ID requested
⏵ 1	2022-02-17 08:59:18	announced

Vale, č, ka J, Camosseto V, McEwan DG, Terawaki S, Liu Z, Strock E, Almeida CR, Su B, Dikic I, Liang Y, Gatti E, Pierre P, RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages. R Soc Open Sci, 8(7):202333(2021) [pubmed]

submitter keyword: Rufy4, Mitophagy,SKP, alveolar macrophages, LPS

Ivan dikic
contact affiliation	Institute of biochemistry 2, Goethe University.
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Anshu Bhattacharya
contact affiliation	Goethe University, Institute of Biochemistry 2, Buchmann Institute For Molecular Life Sciences
contact email	bhattacharya@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026728

PRIDE project URI

• PRIDE
  1. PXD026728
     1. Label: PRIDE project
     2. Name: Rufy4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages