PXD026723

PXD026723 is an original dataset announced via ProteomeXchange.

Title	The cell wall proteome of Craterostigma plantagineum cell cultures habituated to dichlobenil and isoxaben
Description	The remarkable desiccation tolerance of the vegetative tissues in the resurrection species Craterostigma plantagineum (Hochst.) is favored by its unique cell wall folding mechanism that allows the ordered and reversible shrinking of the cells without damaging neither the cell wall nor the underlying plasma membrane. The ability to withstand extreme drought is also maintained in abscisic acid pre-treated calli, which can be cultured both on solid and in liquid culture media. Cell wall research has greatly advanced thanks to the use of inhibitors affecting the biosynthesis of e.g. cellulose, since they allowed the identification of the compensatory mechanisms underlying habituation. Considering the innate cell wall plasticity of C. plantagineum, the goal of this investigation was to understand whether habituation to the cellulose biosynthesis inhibitors dichlobenil and isoxaben entailed or not identical mechanisms as known for non-resurrection species and to decipher the cell wall proteome of habituated cells. The results showed that exposure of C. plantagineum calli/cells triggered abnormal phenotypes as reported in non-resurrection species. Additionally, the data demonstrated that it was possible to habituate Craterostigma cells to dichlobenil and isoxaben and that gene expression and proteomics did not follow the same trend. Shotgun and gel-based proteomics revealed a common set of proteins induced upon habituation, but also identified candidates solely induced by habituation to one of the two inhibitors. Finally, it is hypothesized that alterations in auxin levels are responsible for the increased abundance of cell wall-related proteins upon habituation.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:36:11.875.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Craterostigma plantagineum; NCBI TaxID: 4153;
ModificationList	L-kynurenine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 14:32:46	ID requested
⏵ 1	2022-02-17 04:36:12	announced

Guerriero G, Achen C, Xu X, Planchon S, Leclercq CC, Sergeant K, Berni R, Hausman JF, Renaut J, Legay S, Cell Cultures Habituated to Dichlobenil and Isoxaben. Cells, 10(9):(2021) [pubmed]

submitter keyword: Craterostigma plantagineum, dichlobenil, isoxaben, habituation, cell wall, qPCR, proteomics

Gea Guerriero
contact affiliation	Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 41, Rue du Brill, L-4422 Belvaux, Luxembourg
contact email	gea.guerriero@list.lu
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026723
     1. Label: PRIDE project
     2. Name: The cell wall proteome of Craterostigma plantagineum cell cultures habituated to dichlobenil and isoxaben