PXD026716

PXD026716 is an original dataset announced via ProteomeXchange.

Title	Defining the RBPome of T helper cells to study higher order post-transcriptional gene 1 regulation
Description	Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This becomes evident for the Icos mRNA encoding an essential costimulatory receptorthatexhibits coordinate regulation by several RNA-binding proteins (RBPs), including Roquin-1 and Roquin-2. Utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS) we identify a core RBPome of 798 mouse and 801 human T cell proteins. The RBPome also contained Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we selected ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also unraveled Roquin-1/2-dependent and target-specific coregulation of Icos byCelf1 and Igf2bp3. These findings define the cellular RBPome as post-transcriptional context that predetermines the extent of target regulation by individual trans-acting factors.
HostingRepository	PRIDE
AnnounceDate	2021-06-28
AnnouncementXML	Submission_2021-06-28_01:16:36.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 08:11:13	ID requested
⏵ 1	2021-06-28 01:16:36	announced

Dataset with its publication pending

submitter keyword: Roquin, Bio-ID, RBPome

Vigo Heissmeyer
contact affiliation	Research Unit Molecular Immune Regulation, Helmholtz Center Munich, Munich, Germany; Institute for Immunology, Biomedical Center, Ludwig-Maximilians-Universität in Munich, Planegg-Martinsried, Germany
contact email	vigo.heissmeyer@med.uni-muenchen.de
lab head
Juliane Merl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026716

PRIDE project URI

• PRIDE
  1. PXD026716
     1. Label: PRIDE project
     2. Name: Defining the RBPome of T helper cells to study higher order post-transcriptional gene 1 regulation