PXD026713

PXD026713 is an original dataset announced via ProteomeXchange.

Title	Dynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings
Description	The project aimed to create dynamic maps of protein-protein-metabolite complexes of Arabidopsis thaliana seedlings using PROMIS (PROtein–Metabolite Interactions using Size separation). The approach involves using size exclusion chromatography (SEC) to separate complexes, followed by LC-MS-based proteomics and metabolomics analysis of the obtained fractions. Co-elution is used to reconstruct the protein-metabolite interactions (PMIs) networks. PROMIS strongly progresses understanding protein-small molecule interactions due to its non-targeted manner, cell-wide scale, and generic nature, making it suitable across biological systems. Combining PROMIS with mashing learning approach SLIMP “supervised learning of metabolite-protein interactions from multiple co-fractionation mass spectrometry datasets” allows computing a global map of metabolite-protein interactions in vivo.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:59:33.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ewelina Sokolowska
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-15 06:07:41	ID requested
1	2022-10-14 05:32:39	announced
⏵ 2	2023-11-14 07:59:34	announced	2023-11-14: Updated project metadata.

Palos K, Nelson Dittrich AC, Yu L, Brock JR, Railey CE, Wu HL, Sokolowska E, Skirycz A, Hsu PY, Gregory BD, Lyons E, Beilstein MA, Nelson ADL, Identification and functional annotation of long intergenic non-coding RNAs in Brassicaceae. Plant Cell, 34(9):3233-3260(2022) [pubmed]

submitter keyword: protein-metabolite complexes, ligand,protein-protein complexes

Aleksandra Skirycz
contact affiliation	Max-Planck-Institut für Molekulare Pflanzenphysiologie
contact email	Skirycz@mpimp-golm.mpg.de
lab head
Ewelina Sokolowska
contact affiliation	Max Planck Institute of Molecular Plant Physiology Wissenschaftspark Am Mühlenberg 1 14476 Potsdam-Golm
contact email	esokolowska@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD026713

PRIDE project URI

• PRIDE
  1. PXD026713
     1. Label: PRIDE project
     2. Name: Dynamic maps of protein-protein-metabolite complexes of Arabidopsis Thaliana seedlings