PXD026704

PXD026704 is an original dataset announced via ProteomeXchange.

Title	Coating and corruption of human neutrophils by bacterial outer membrane vesicles
Description	Porphyromonas gingivalis is a keystone oral pathogen that successfully manipulates the human innate immune defenses, resulting in a chronic pro-inflammatory state of periodontal tissues and beyond. Here we demonstrate that secreted outer membrane vesicles (OMVs) are deployed by P. gingivalis to selectively coat and activate human neutrophils, thereby provoking degranulation without neutrophil killing. Secreted granule components with antibacterial activity, especially LL-37 and MPO, are subsequently degraded by potent OMV-bound proteases known as gingipains, thereby ensuring bacterial survival. In contrast to neutrophils, the P. gingivalis OMVs are efficiently internalized by macrophages and epithelial cells. Importantly, we show that neutrophil coating is a conserved feature displayed by OMVs of at least one other oral pathogen, namely Aggregatibacter actinomycetemcomitans. Altogether, we conclude that P. gingivalis deploys its OMVs for a neutrophil-deceptive strategy to escape phagocytosis and to create a favorable inflammatory niche.
HostingRepository	PRIDE
AnnounceDate	2022-08-16
AnnouncementXML	Submission_2022-08-16_13:14:47.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Hirschfeld
SpeciesList	scientific name: Porphyromonas gingivalis W83; NCBI TaxID: 242619; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue; L-citrulline
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 23:50:41	ID requested
⏵ 1	2022-08-16 13:14:47	announced

Dataset with its publication pending

submitter keyword: OMVs, Porphyromonas gingivalis, neutrophils, myeloperoxidase, citrullination, gingipains, LL-37

Prof. Dr. Dörte Becher
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Claudia Hirschfeld
contact affiliation	University of Greifswald
contact email	claudia.hirschfeld@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD026704

PRIDE project URI

• PRIDE
  1. PXD026704
     1. Label: PRIDE project
     2. Name: Coating and corruption of human neutrophils by bacterial outer membrane vesicles