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PXD026700

PXD026700 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGrowth Rate-Dependent Coordination of Catabolism and Anabolism in the Archaeon Methanococcus maripaludis under Phosphate Limitation
DescriptionCatabolic and anabolic processes are finely coordinated in microbes to provide optimized fitness under varying environmental conditions. Understanding this coordination and the resulting physiological traits identifies fundamental microbial strategies of acclimation. Here, we characterized the systems-level physiology of Methanococcus maripaludis, a niche-specialized methanogenic archaeon, at different growth rates under phosphate (i.e., anabolic) limitation. We observed a decoupling of catabolism and anabolism resulting in lower biomass yield relative to catabolically limited cells. In addition, the mass abundance of several coarse-grained proteome sectors exhibited a linear relationship with growth rate, most notably ribosomes and their biogenesis. Accordingly, cellular RNA content also correlated with growth rate. Although the methanogenesis proteome sector was invariant, the metabolic capacity for methanogenesis correlated with growth rate suggesting translationally independent regulation. These observations are in stark contrast to the physiology of M. maripaludis under formate (i.e., catabolic) limitation, where cells keep an invariant proteome including ribosomal content and a high methanogenesis capacity across a wide range of growth rates. Our findings reveal that although highly niche-specialized, M. maripaludis employs fundamentally different strategies to coordinate global physiology during anabolic phosphate and catabolic formate limitation.
HostingRepositoryMassIVE
AnnounceDate2022-06-15
AnnouncementXMLSubmission_2022-06-15_20:13:02.383.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVadim Patsalo
SpeciesList scientific name: Methanococcus maripaludis S2; NCBI TaxID: 267377;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-14 20:32:23ID requested
12022-06-15 20:13:02announced
Publication List
no publication
Keyword List
submitter keyword: methanogen, methanococcus maripaludis, formate, phosphate, archaea, catabolism, metabolism, anabolism
Contact List
James R. Williamson
contact affiliationThe Scripps Research Institute
contact emailjrwill@scripps.edu
lab head
Alfred M. Spormann
contact affiliationStanford University
contact emailspormann@stanford.edu
lab head
Vadim Patsalo
contact affiliationScripps Research
contact emailpatsalov@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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