PXD026691

PXD026691 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of GBA iPSC-dopamine neurons
Description	Heterozygous mutations in the glucocerebrosidase gene (GBA) are the strongest common genetic risk factors for Parkinson’s disease (PD) present in around 5-10% of PD patients, resulting in lower age of onset and exacerbating disease progression, including an increased risk of dementia. However, the exact mechanisms leading from dysfunction of the enzyme glucocerebrosidase (GCase) encoded by GBA to PD pathogenesis and neurodegeneration remain unclear. Applying a novel multi-part enrichment workflow we have developed, we have isolated and quantified peptides with phosphorylation, cysteine-modification or N-linked glycosylation simultaneously. We applied this methodology to iPSC-derived dopaminergic neurons from GBA-N370S PD patients and healthy age-matched controls, identifying large numbers of dysregulated native and modified proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:08.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pia Jensen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine; phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 05:58:03	ID requested
1	2023-03-04 13:29:28	announced
⏵ 2	2023-11-14 08:53:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Neuritogenesis/Parkinson’s/Proteomics/Stem cells/Glucocerebrosidase/post-translational modification

Richard Wade-Martins
contact affiliation	Lab head, Oxford Parkinson's Disease Centre, Department of Physiology, Anatomy and Genetics, University of Oxford, OX1 3QX, Oxford, UK
contact email	richard.wade-martins@dpag.ox.ac.uk
lab head
Pia Jensen
contact affiliation	University of Southern Denmark
contact email	pjensen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD026691

PRIDE project URI

• PRIDE
  1. PXD026691
     1. Label: PRIDE project
     2. Name: Proteomic analysis of GBA iPSC-dopamine neurons