PXD026687

PXD026687 is an original dataset announced via ProteomeXchange.

Title	stem cells from human exfoliated deciduous teeth cultured with human platelet lysate
Description	Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients, especially fetal bovine serum, will increase the risk of zoonotic disease transmission during the use of animal-derived products. Human-platelet-lysate (hPL) was used in human cell expansion with reliability in clinical applications. This study aimed to explore the feasibility and effect of HPL in the cultivation of SHED. We use proteomics to interpret the impact of HPL on the protein profile of SHED. Propagation, differentiation, qPCR and immunofluorescence were used to detect the effect of HPL on SHED.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_00:46:49.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tong Lei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 05:35:30	ID requested
⏵ 1	2022-05-19 00:46:50	announced

Lei T, Liu Y, Deng S, Xiao Z, Yang Y, Zhang X, Bi W, Du H, Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells. J Nanobiotechnology, 20(1):176(2022) [pubmed]

submitter keyword: SHED, HPL, proteomics

Hongwu Du
contact affiliation	School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
contact email	hongwudu@ustb.edu.cn
lab head
Tong Lei
contact affiliation	University of science and technology beijing
contact email	1943579770@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD026687

PRIDE project URI

• PRIDE
  1. PXD026687
     1. Label: PRIDE project
     2. Name: stem cells from human exfoliated deciduous teeth cultured with human platelet lysate