PXD026684

PXD026684 is an original dataset announced via ProteomeXchange.

Title	mNaa10-KO liver immunoprecipitation
Description	NAA10-mediated N-terminal acetylation is widespread and has been considered essential for viability in many model organisms. However, a Naa10 null mouse model is viable and has intact global N-terminal acetylation levels. The purpose of this project was to assess the N-terminal acetyltransferase activity of NAA15 immunoprecipitates from Naa10-KO mouse liver. We show that there is a novel paralog of Naa10 present in mice, termed Naa12, with a NatA-type enzymatic activity. We demonstrate the presence of Naa12 by detecting unique Naa12 peptides in NAA15 immunoprecipitates.
HostingRepository	PRIDE
AnnounceDate	2021-08-18
AnnouncementXML	Submission_2021-08-17_22:27:23.382.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rasmus Ree
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-14 05:35:18	ID requested
1	2021-08-17 13:14:03	announced
⏵ 2	2021-08-17 22:27:24	announced	2021-08-18: Updated project metadata.

10.7554/ELIFE.65952;

Kweon HY, Lee MN, Dorfel M, Seo S, Gottlieb L, PaPazyan T, McTiernan N, Ree R, Bolton D, Garcia A, Flory M, Crain J, Sebold A, Lyons S, Ismail A, Marchi E, Sonn SK, Jeong SJ, Jeon S, Ju S, Conway SJ, Kim T, Kim HS, Lee C, Roh TY, Arnesen T, Marmorstein R, Oh GT, Lyon GJ, in mice in the amino-terminal acetylation pathway. Elife, 10():(2021) [pubmed]

submitter keyword: N-terminal acetylation, NTA,Naa10, Naa12, NAT

Thomas Arnesen
contact affiliation	University of Bergen, Department of Biomedicine
contact email	thomas.arnesen@uib.no
lab head
Rasmus Ree
contact affiliation	University of Bergen
contact email	rasmus.ree@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026684

PRIDE project URI

• PRIDE
  1. PXD026684
     1. Label: PRIDE project
     2. Name: mNaa10-KO liver immunoprecipitation