PXD026664

PXD026664 is an original dataset announced via ProteomeXchange.

Title	Cyclotides isolated from violet plants of Cameroon are inhibitors of human prolyl oligopeptidase
Description	Traditional medicine and the use of herbal remedies are well established in the African health care system. For instance, Violaceae plants are used for antimicrobial or anti-inflammatory applications in folk medicine. This study describes the phytochemical analysis and bioactivity screening of four species of the violet tribe Allexis found in Cameroon. Allexis cauliflora, Allexis obonensis, Allexis batangae and Allexis zygomorpha were evaluated for the expression of circular peptides (cyclotides) by mass spectrometry. The unique cyclic cystine-rich motif was identified in several peptides of all four species. Knowing that members of this peptide family are protease inhibitors, the plant extracts were evaluated for the inhibition of human prolyl oligopeptidase (POP). Since all four species inhibited POP activity, a bioactivity-guided fraction approach was performed to isolate peptide inhibitors. These novel cyclotides, alca 1 and alca 2 exhibited IC50 values of 8.5 and 4.4 µM, respectively. To obtain their amino acid sequence information, combinatorial enzymatic proteolysis was performed. The proteolytic fragments were evaluated in MS/MS fragmentation experiments and the full-length amino acid sequences were obtained by de novo annotation of fragment ions. In summary, this study identified inhibitors of the human protease POP, which is a drug target for inflammatory or neurodegenerative disorders.
HostingRepository	PRIDE
AnnounceDate	2021-08-09
AnnouncementXML	Submission_2021-08-09_03:51:12.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roland Hellinger
SpeciesList	scientific name: Allexis; NCBI TaxID: 317447;
ModificationList	No PTMs are included in the dataset
Instrument	MALDI Solutions; Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-11 08:38:13	ID requested
⏵ 1	2021-08-09 03:51:13	announced

Gattringer J, Ndogo OE, Retzl B, Ebermann C, Gruber CW, Hellinger R, Cyclotides Isolated From Violet Plants of Cameroon Are Inhibitors of Human Prolyl Oligopeptidase. Front Pharmacol, 12():707596(2021) [pubmed]

submitter keyword: cyclic cystine-rich peptides, prolyl oligopeptidase (POP), Allexis cauliflora, peptide, protease inhibitor

Roland Hellinger
contact affiliation	Medical University of Vienna Center for Physiology and Pharmacology
contact email	roland.hellinger@meduniwien.ac.at
lab head
Roland Hellinger
contact affiliation	Medical University of Vienna, Center for Pharmacology and Physiology
contact email	roland.hellinger@meduniwien.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026664
     1. Label: PRIDE project
     2. Name: Cyclotides isolated from violet plants of Cameroon are inhibitors of human prolyl oligopeptidase