PXD026659 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AATF interactome in human Kms27 cells |
Description | Multiple myeloma is a hematological neoplasm of plasma cells characterized by an abnormal production of immunoglobulins. Che-1/AATF (Che-1) is an RNA binding protein involved in transcription regulation and highly expressed in this malignancy. In order to better characterize its functions in this type of disease, we performed co-immunoprecipitation experiments coupled with a mass-spec analysis by using total MM Kms27 cell lysates. From this analysis we were able to identify more than 600 proteins significantly enriched in Che-1 precipitates. As expected from its role in ribosomal RNA maturation, the Che-1 interactome contains numerous proteins involved in pre-rRNA processing and several ribosomal proteins. Notably, among other proteins interacting with Che-1, we identified many essential components of the paraspeckle which play an important role in the regulation of gene expression. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:53:07.489.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Prerana Wagle |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-11 07:25:00 | ID requested | |
1 | 2022-08-02 23:44:51 | announced | |
⏵ 2 | 2023-11-14 08:53:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Multiple Myeloma,AATF |
Contact List
Bernhard Schermer |
contact affiliation | Department II of Internal Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne,Germany |
contact email | bernhard.schermer@uk-koeln.de |
lab head | |
Prerana Wagle |
contact affiliation | CECAD Research Center |
contact email | proteomics-facility@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026659
- Label: PRIDE project
- Name: AATF interactome in human Kms27 cells