PXD026654

PXD026654 is an original dataset announced via ProteomeXchange.

Title	Identification of formaldehyde-induced DNA-protein crosslinks by PxP-MS
Description	Compromised enzymatic actions, reactive metabolites, and various chemotherapeutics cause toxic covalent DNA-protein crosslinks (DPCs). Failure to repair DPCs results in genomic instability, premature aging, and tumorigenesis. However, understanding the principles underlying DPC formation and repair has been hampered by a lack of methodologies to study DPCs in human cells. Here, we developed a technique for the Purification of x-linked Proteins (PxP), which allows identification and tracking of enzymatic and nonenzymatic DPCs. By combining PxP with quantitative proteomics, we investigated the nature of DPCs induced by formaldehyde.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:01.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-11 07:24:32	ID requested
1	2023-01-20 06:33:42	announced
⏵ 2	2023-11-14 08:53:01	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: formaldehyde, PxP,DNA-protein crosslinks, SPRTN

Prof. Dr. Julian Stingele
contact affiliation	Gene Center and Department of Biochemistry Ludwig-Maximilians-Universität München Postal Address: Feodor-Lynen-Straße 25 Visiting Address: BioSysM, Butenandtstraße 1
contact email	stingele@genzentrum.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD026654

PRIDE project URI

• PRIDE
  1. PXD026654
     1. Label: PRIDE project
     2. Name: Identification of formaldehyde-induced DNA-protein crosslinks by PxP-MS