PXD026651

PXD026651 is an original dataset announced via ProteomeXchange.

Title	Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
Description	Production of biopharmaceuticals relies on the expression of mammalian cDNAs in host organisms. Here we show that the expression of a human cDNA in the moss Physcomitrium patens generates the expected full-length and four additional transcripts due to unexpected splicing. This mRNA splicing results in non-functional protein isoforms, cellular misallocation of the proteins and low product yields. We integrated these results together with the results of our analysis of all 32,926 protein-encoding Physcomitrella genes and their 87,533 annotated transcripts in a web application, physCO, for automatized optimization. A thus optimized cDNA results in about twelve times more protein, which correctly localizes to the ER. An analysis of codon preferences of different production hosts suggests that similar effects occur also in non-plant hosts. We anticipate that the use of our methodology will prevent so far undetected mRNA heterosplicing resulting in maximized functional protein amounts for basic biology and biotechnology.
HostingRepository	PRIDE
AnnounceDate	2022-01-10
AnnouncementXML	Submission_2022-01-10_10:34:15.083.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026651
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ralf Reski
SpeciesList	scientific name: Physcomitrium patens; NCBI TaxID: 3218;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-11 07:19:52	ID requested
1	2022-01-10 10:28:46	announced
⏵ 2	2022-01-10 10:34:15	announced	2022-01-10: Updated publication reference for PubMed record(s): 34385580.

Top O, Milferstaedt SWL, van Gessel N, Hoernstein SNW, Ö, zdemir B, Decker EL, Reski R, Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms. Commun Biol, 4(1):964(2021) [pubmed]

submitter keyword: aberrant mRNA-splicing, blood coagulation factor IX, heterologous expression systems, Physcomitrium patens

Ralf Reski
contact affiliation	Head, Chair Plant Biotechnology Faculty of Biology University of Freiburg Schaenzlestrasse 1 D-79104 Freiburg Germany
contact email	ralf.reski@biologie.uni-freiburg.de
lab head
Ralf Reski
contact affiliation	Faculty of Biology, University of Freiburg (Chair Plant Biotechnology), Schaenzlestr. 1, D-79104 Freiburg
contact email	ralf.reski@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD026651

PRIDE project URI

• PRIDE
  1. PXD026651
     1. Label: PRIDE project
     2. Name: Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms