PXD026646

PXD026646 is an original dataset announced via ProteomeXchange.

Title	LFQ-proteomic on bbs1 zebrafish photoreceptor outer segments
Description	Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal dystrophy, intellectual disability, polydactyly, obesity and renal anomalies. In photoreceptors (PR), light sensation occurs in outer segments (OSs), which are specialized primary cilia. BBS1, the major BBS gene, is part of a protein complex called “BBSome”, which is involved in intracellular protein transport. However, the precise function of BBS1/BBSome in controlling trafficking of ciliary proteins in PRs remains unclear. To investigate the role of the BBSome in photoreceptors, we conducted a label free quantitative proteomic investigation on the protein contend of isolated adult mutant and control outer segments. We revealed that a Bbs1 KO results in the loss of the entire BBSome from OSs. Besides the loss of the BBSome we found an overall accumulation of non-outer segment proteins in the OS. A majority of these proteins were membrane-associated, supporting the role of BBS1/the BBSome in controlling ciliary transport of membrane-associated proteins.
HostingRepository	PRIDE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_14:28:28.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Masek
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-11 06:28:56	ID requested
⏵ 1	2022-02-10 14:28:28	announced

Dataset with its publication pending

submitter keyword: Zebrafish, Photoreceptor, Outer segment, LFQ-P, BBS

Ruxandra Bachmann- Gagescu
contact affiliation	Institute of Medical Genetics, Bachmann-Lab, University of Zurich
contact email	ruxandra.bachmann@mls.uzh.ch
lab head
Markus Masek
contact affiliation	University of Zurich
contact email	markus.masek@uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026646

PRIDE project URI

• PRIDE
  1. PXD026646
     1. Label: PRIDE project
     2. Name: LFQ-proteomic on bbs1 zebrafish photoreceptor outer segments