PXD026643

PXD026643 is an original dataset announced via ProteomeXchange.

Title	Salt-inhibited protein 2-hydroxyisobutyrylation affects silique development of Arabidopsis thaliana
Description	Lysine 2-hydroxyisobutyrylation (Khib) is one of the newly discovered post-translational modifications (PTMs) through protein acylation. It has been reported to be widely distribute in both eukaryotes and prokaryotes, and plays an important role in chromatin conformation change, gene transcription, subcellular localization, protein-protein interaction, signal transduction, and cellular proliferation. In this study, we compared the siliques from Arabidopsis thaliana under salt stress (Ss) with those in the control (Cs). The results showed that this highly conserved modification was abundant in siliques. However, there were certain significant differences between the Ss and the Cs: 3810 normalized 2-hydroxyisobutyrylation sites on 1254 proteins were identified in siliques under salt stress, and lysine 2-hydroxyisobutyrylation was up-regulated at 96 sites on 78 proteins while down-regulated at 282 sites on 205 proteins in Ss. In the KEGG pathway enrichment analysis, Khib-modified proteins were enriched in several pathways related to energy metabolism, including gluconeogenesis pathway, pentose phosphate pathway, and pyruvate metabolism. Overall, our work reveals the first systematic analysis of Khib proteome in Arabidopsis siliques under salt stress, and sheds a light on the future studies on the regulatory mechanisms of Khib during the salt stress response of plants.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:24:53.821.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Geriqiqige Hong
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TMT quantitation analysis

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-11 03:55:17	ID requested
⏵ 1	2022-02-17 04:24:54	announced

Hong G, Su X, Xu K, Liu B, Wang G, Li J, Wang R, Zhu M, Li G, Salt stress downregulates 2-hydroxybutyrylation in Arabidopsis siliques. J Proteomics, 250():104383(2022) [pubmed]

submitter keyword: Arabidopsis thaliana. Silique. Lysine 2-hydroxyisobutyrylation. Salt stress

Guojing Li
contact affiliation	Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University
contact email	liguojing@imau.edu.cn
lab head
Geriqiqige Hong
contact affiliation	Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University
contact email	honggeriqiqige@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD026643

PRIDE project URI

• PRIDE
  1. PXD026643
     1. Label: PRIDE project
     2. Name: Salt-inhibited protein 2-hydroxyisobutyrylation affects silique development of Arabidopsis thaliana