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PXD026641

PXD026641 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHypoxia induces a transcriptional primitive streak signature in ES cells and enhances lineage patterning in gastruloids
DescriptionThe cellular microenvironment shapes stem cell identity and differentiation capacity. Mammalian early embryos are exposed to hypoxia in vivo and benefit from hypoxic culture in vitro. Yet, how different components of the hypoxia response impact stem cell transcriptional networks and lineage choices remains unclear. Here we investigated the effect of acute and prolonged hypoxia on stem cell states and differentiation efficiencies of embryonic and extraembryonic cells. We show that prolonged hypoxia enhances differentiation of embryonic stem (ES) cells towards the mesendoderm lineage by transcriptionally priming cells with a primitive streak signature including Wnt3 and T expression. Exposure to hypoxia in ES culture or during formation of gastrulation-mimicking organoids (gastruloids) moderates T expression and enhances structural complexity. Hypoxic gastruloids generated without exogenous Wnt induction can spontaneously elongate and self-organize. Direct gene regulation by Hif1a, combined with DNA demethylation and metabolic rewiring modulate the transcriptional response and phenotypic outcome. Our findings highlight the influence of the microenvironment on stem cell function and provide a rationale supportive of applying physiological conditions in synthetic embryo models.
HostingRepositoryPRIDE
AnnounceDate2022-09-05
AnnouncementXMLSubmission_2022-09-05_02:48:14.992.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Meierhofer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-11 02:56:20ID requested
12022-09-05 02:48:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: early embryos, hypoxia, embryonic stem
Contact List
Aydan Bulut-Karslioglu
contact affiliationSofja Kovaleskaja Research Group, Max Planck Institute for Molecular Genetics, Berlin, Germany
contact emailKarsliog@molgen.mpg.de
lab head
David Meierhofer
contact affiliationMass Spectrometry Facility MPIMG
contact emailmeierhof@molgen.mpg.de
dataset submitter
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Dataset FTP location
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