PXD026636

PXD026636 is an original dataset announced via ProteomeXchange.

Title	Translation machinery for deliberate mistranslation of the genetic code in Bacteria
Description	“Inaccurate” expression of the genetic code, also known as mistranslation, is an emerging paradigm in microbial studies. Growing evidence indicates that many microbial pathogens can deliberately mistranslate their genetic code to help invade a host or evade host immune responses. However, discovering new capacities for deliberate mistranslation remains a challenge because each group of pathogens typically employs a unique mistranslation mechanism. In this study, we address this problem by studying duplicated genes of aminoacyl-tRNA synthetases. Using bacterial prolyl-tRNA synthe¬tase (ProRS) genes as an example, we identify an anomalous ProRS isoform, ProRSx, and a corre¬sponding tRNA, tRNAProA, that are predominately found in plant pathogens from Streptomyces species. We then show that tRNAProA has an unusual hybrid structure that allows this tRNA to mistranslate alanine codons as proline. We finally provide biochemical, genetic, and mass-spectro-metric evidence that cells which express ProRSx and tRNAProA can translate GCU alanine codons both as alanine and proline. This dual use of alanine codons creates a hidden proteome diversity due to stochastic Ala→Pro mutations in protein sequences. Thus, we show that important plant pathogens are equipped with a tool to alter the identity of their sense codons. This finding reveals the first example of a natural tRNA synthetase/tRNA pair for dedicated mistranslation of sense codons.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:37:23.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kyle Hoffman
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 22:45:39	ID requested
⏵ 1	2022-02-22 04:37:24	announced

Vargas-Rodriguez O, Badran AH, Hoffman KS, Chen M, Crnkovi, ć A, Ding Y, Krieger JR, Westhof E, S, ö, ll D, Melnikov S, Bacterial translation machinery for deliberate mistranslation of the genetic code. Proc Natl Acad Sci U S A, 118(35):(2021) [pubmed]

submitter keyword: genetic code, tRNA, aminoacyl-tRNA synthetase, mistranslation

Professor Dieter Soll
contact affiliation	Department of Molecular Biophysics and Biochemistry Department of Chemistry Yale University New Haven, CT USA
contact email	dieter.soll@yale.edu
lab head
Kyle Hoffman
contact affiliation	Bioinformatics Solutions Inc.
contact email	khoffman@bioinfor.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026636
     1. Label: PRIDE project
     2. Name: Translation machinery for deliberate mistranslation of the genetic code in Bacteria