PXD026633

PXD026633 is an original dataset announced via ProteomeXchange.

Title	A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
Description	This study tracks the proteome during recovery from 10 kGy acute ionizing radiation (IR) in Deinococcus radiodurans R1 (WT). After 1 hour of recovery post-IR exposure, we observed 37 proteins significantly differentially expressed, including several within the Radiation and Dessication Response (RDR) pathway. Additionally, we also explored the regulatory network of a sRNA named PprS (previously Dsr2) in Deinococcus radiodurans by comparing the proteome of a sRNA knockdown strain (PprSKD, which demonstrates a ~2-fold decrease in PprS expression) to WT D. radiodurans during unirradiated conditions at late-exponential phase. Comparison between these two strains demonstrated decreased levels of one of PprS's targets, PprM, in the PprSKD strain which validated the activation mechanism we propose for PprS on pprM.
HostingRepository	PRIDE
AnnounceDate	2021-06-25
AnnouncementXML	Submission_2021-06-24_23:14:27.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordan Villa
SpeciesList	scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 22:31:55	ID requested
⏵ 1	2021-06-24 23:14:28	announced

Dataset with its publication pending

submitter keyword: deinococcus, sRNA, ionizing radiation, IR, deinococcus radiodurans, shotgun proteomics, timecourse

Lydia M Contreras
contact affiliation	McKetta Department of Chemical Engineering, The University of Texas at Austin, USA
contact email	lcontrer@che.utexas.edu
lab head
Jordan Villa
contact affiliation	University of Texas at Austin
contact email	jkvilla27@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026633

PRIDE project URI

• PRIDE
  1. PXD026633
     1. Label: PRIDE project
     2. Name: A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation