PXD026623 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Finding New Drug Targets for Niemann Pick Type C Hepatic Disease Based On Proteomic Network Analysis |
Description | A significant area of rare diseases research is the investigation of druggable protein encoding genes that contribute to pathogenesis. Niemann Pick Type C (NPC) disease can be considered as a challenging one, among all rare diseases, because it has been associated with a poor prognosis and an unclear molecular pathogenesis. In recent years, Proteomics analysis has become a functional and useful technology for profiling protein expression and find possible drugs targets. In the present study, hepatocytes derived from wild type and Npc1 deficient mice were analyzed by mass spectrometry-based proteomics, followed by pathway analysis and statistical interpretation, performed with the QIAGEN Ingenuity Pathway Analysis (IPA) software. Applications for protein function was built using IPA and gene ontology analysis. We identified and reliably quantified a total of 3833 proteins, among them 416 presented a significant p-value (<0.05) being classified 149 as upregulated (log2 fold change >1) and 6 as downregulated (Log2 fold change <-1). Our analysis revealed that the most significant changed proteins are related to liver damage, lipid metabolism and inflammatory response. In addition, in the group of up/downregulated proteins, 47 proteins were identified as lysosomal proteins and 22 as mitochondrial proteins. Importantly, we found that of those proteins CTSB, LIPA, DPP7, GLMP and DECR1 are related to liver fibrosis, liver damage and steatosis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-23 |
AnnouncementXML | Submission_2021-08-22_23:44:45.780.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD026623 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Robert Hardt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-10 09:04:34 | ID requested | |
⏵ 1 | 2021-08-22 23:44:46 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Liver, Niemann Pick Type C, NPC, LC-MS, Hepatocytes |
Contact List
Dominic Winter |
contact affiliation | Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Germany |
contact email | dominic.winter@uni-bonn.de |
lab head | |
Robert Hardt |
contact affiliation | IBMB - University of Bonn |
contact email | rhardt@uni-bonn.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026623
- Label: PRIDE project
- Name: Finding New Drug Targets for Niemann Pick Type C Hepatic Disease Based On Proteomic Network Analysis