PXD026623

PXD026623 is an original dataset announced via ProteomeXchange.

Title	Finding New Drug Targets for Niemann Pick Type C Hepatic Disease Based On Proteomic Network Analysis
Description	A significant area of rare diseases research is the investigation of druggable protein encoding genes that contribute to pathogenesis. Niemann Pick Type C (NPC) disease can be considered as a challenging one, among all rare diseases, because it has been associated with a poor prognosis and an unclear molecular pathogenesis. In recent years, Proteomics analysis has become a functional and useful technology for profiling protein expression and find possible drugs targets. In the present study, hepatocytes derived from wild type and Npc1 deficient mice were analyzed by mass spectrometry-based proteomics, followed by pathway analysis and statistical interpretation, performed with the QIAGEN Ingenuity Pathway Analysis (IPA) software. Applications for protein function was built using IPA and gene ontology analysis. We identified and reliably quantified a total of 3833 proteins, among them 416 presented a significant p-value (<0.05) being classified 149 as upregulated (log2 fold change >1) and 6 as downregulated (Log2 fold change <-1). Our analysis revealed that the most significant changed proteins are related to liver damage, lipid metabolism and inflammatory response. In addition, in the group of up/downregulated proteins, 47 proteins were identified as lysosomal proteins and 22 as mitochondrial proteins. Importantly, we found that of those proteins CTSB, LIPA, DPP7, GLMP and DECR1 are related to liver fibrosis, liver damage and steatosis.
HostingRepository	PRIDE
AnnounceDate	2021-08-23
AnnouncementXML	Submission_2021-08-22_23:44:45.780.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026623
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Hardt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 09:04:34	ID requested
⏵ 1	2021-08-22 23:44:46	announced

Dataset with its publication pending

submitter keyword: Liver, Niemann Pick Type C, NPC, LC-MS, Hepatocytes

Dominic Winter
contact affiliation	Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Germany
contact email	dominic.winter@uni-bonn.de
lab head
Robert Hardt
contact affiliation	IBMB - University of Bonn
contact email	rhardt@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026623
     1. Label: PRIDE project
     2. Name: Finding New Drug Targets for Niemann Pick Type C Hepatic Disease Based On Proteomic Network Analysis