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PXD026623

PXD026623 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFinding New Drug Targets for Niemann Pick Type C Hepatic Disease Based On Proteomic Network Analysis
DescriptionA significant area of rare diseases research is the investigation of druggable protein encoding genes that contribute to pathogenesis. Niemann Pick Type C (NPC) disease can be considered as a challenging one, among all rare diseases, because it has been associated with a poor prognosis and an unclear molecular pathogenesis. In recent years, Proteomics analysis has become a functional and useful technology for profiling protein expression and find possible drugs targets. In the present study, hepatocytes derived from wild type and Npc1 deficient mice were analyzed by mass spectrometry-based proteomics, followed by pathway analysis and statistical interpretation, performed with the QIAGEN Ingenuity Pathway Analysis (IPA) software. Applications for protein function was built using IPA and gene ontology analysis. We identified and reliably quantified a total of 3833 proteins, among them 416 presented a significant p-value (<0.05) being classified 149 as upregulated (log2 fold change >1) and 6 as downregulated (Log2 fold change <-1). Our analysis revealed that the most significant changed proteins are related to liver damage, lipid metabolism and inflammatory response. In addition, in the group of up/downregulated proteins, 47 proteins were identified as lysosomal proteins and 22 as mitochondrial proteins. Importantly, we found that of those proteins CTSB, LIPA, DPP7, GLMP and DECR1 are related to liver fibrosis, liver damage and steatosis.
HostingRepositoryPRIDE
AnnounceDate2021-08-23
AnnouncementXMLSubmission_2021-08-22_23:44:45.780.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026623
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRobert Hardt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-10 09:04:34ID requested
12021-08-22 23:44:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Liver, Niemann Pick Type C, NPC, LC-MS, Hepatocytes
Contact List
Dominic Winter
contact affiliationInstitute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Germany
contact emaildominic.winter@uni-bonn.de
lab head
Robert Hardt
contact affiliationIBMB - University of Bonn
contact emailrhardt@uni-bonn.de
dataset submitter
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Dataset FTP location
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Repository Record List
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