PXD026603

PXD026603 is an original dataset announced via ProteomeXchange.

Title	XL-MS Analysis of Recombinant GPR158:RGS7:Gb5 Complex in Detergent Micelles
Description	GPR158 is widely expressed in the brain and have been implicated in depression, cognition, mood and several cancers. GPR158 is also involved in retinal photoreceptor signaling along with it involvement in synaptic organization. GPR158 interact with G-protein regulator-RGS7 and brings it to plasma membrane to allosterically modulate GTPase activity of G-protein alpha subunit. However, the signaling mechanism and it structural organization is not known. The mnuscript associated with this deposition describes a structural analysis of GPR158 in complex with downstream effectors RGS7 and Gb5. This deposition pertains to the crosslinking mass spectrometry experiments conducted to complement these efforts.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:27.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timothy Strutzenberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-09 22:54:42	ID requested
1	2023-03-10 23:32:41	announced
⏵ 2	2023-11-14 08:38:29	announced	2023-11-14: Updated project metadata.

Patil DN, Singh S, Laboute T, Strutzenberg TS, Qiu X, Wu D, Novick SJ, Robinson CV, Griffin PR, Hunt JF, Izard T, Singh AK, Martemyanov KA, 5 signaling complex. Science, 375(6576):86-91(2022) [pubmed]

submitter keyword: RGS7, orphan GPCR, Gb5, GPCR-RGS complex, Membrane protein complex, GPR158, G-protein coupled receptor,Chemical Crosslinking

Patrick R. Griffin
contact affiliation	Department of Molecular Medicine The Scripps Research Institute
contact email	pgriffin@scripps.edu
lab head
Timothy Strutzenberg
contact affiliation	TSRI
contact email	tstrutze@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD026603

PRIDE project URI

• PRIDE
  1. PXD026603
     1. Label: PRIDE project
     2. Name: XL-MS Analysis of Recombinant GPR158:RGS7:Gb5 Complex in Detergent Micelles