PXD026589

PXD026589 is an original dataset announced via ProteomeXchange.

Title	Plasma from Rainbow trout housed for 7 days in normoxic and hypoxic water
Description	Hypoxic water had 5 mg/L of Dissolved Oxygen (DO) and normoxic water had 11.5 mg/L DO. 40 immature rainbow trout (about 100 g) were housed for 7 days under these conditions (20 fish in each treatment). Then fish were anesthetized with 100 mg/L MS-222, and blood was sampled using caudal puncture with heparinized syringes. The blood was centrifuged at 10 000 x g for 4 minutes in Heraeus Fresco 21 centrifuge (Thermo Fisher). Plasma was removed from this using a pipette, and placed in cryotubes, flash frozen in liquid nitrogen, and then stored at -80oC until preparation for proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2021-06-18
AnnouncementXML	Submission_2021-06-17_21:33:48.430.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denina Simmons
SpeciesList	scientific name: Oncorhynchus mykiss; NCBI TaxID: 8022;
ModificationList	acetylated residue
Instrument	6530A Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-09 01:27:19	ID requested
⏵ 1	2021-06-17 21:33:49	announced

L, é, ger JAD, Athanasio CG, Zhera A, Chauhan MF, Simmons DBD, Hypoxic responses in Oncorhynchus mykiss involve angiogenesis, lipid, and lactate metabolism, which may be triggered by the cortisol stress response and epigenetic methylation. Comp Biochem Physiol Part D Genomics Proteomics, 39():100860(2021) [pubmed]

submitter keyword: Plasma, LC-MS, Rainbow Trout, Agilent 6530 QTOF, Shotgun

Dr, Denina Simmons
contact affiliation	Faculty of Science, Ontario Tech University Oshawa, ON, Canada
contact email	denina.simmons@ontariotechu.ca
lab head
Denina Simmons
contact affiliation	Ontario Tech University
contact email	denina.simmons@uoit.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026589

PRIDE project URI

• PRIDE
  1. PXD026589
     1. Label: PRIDE project
     2. Name: Plasma from Rainbow trout housed for 7 days in normoxic and hypoxic water