PXD026576

PXD026576 is an original dataset announced via ProteomeXchange.

Title	Perturbations of the proteome in primary astrocytes from the hSOD1(G93A) ALS mouse model
Description	Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease whose pathophysiology is largely unknown. Despite motor neuron death is recognized as the key event in ALS, astrocytes dysfunctionalities and neuroinflammation were demonstrated to accompany and probably even drive motor neuron loss. Nevertheless, the mechanisms priming astrocyte failure and hyperactivation are still obscure. In this work, altered pathways in ALS astrocytes were unveiled by investigating the proteomic profile of primary spinal-cord astrocytes derived from transgenic ALS mouse model overexpressing the human (h)SOD1(G93A) protein, in comparison with the transgenic counterpart expressing hSOD1(WT) protein. In this research, we showed that hSOD1(G93A) astrocytes present a profound alterations in the expression of proteins involved in proteostasis and glutathione metabolism.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_10:02:22.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Stella
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-08 23:04:19	ID requested
⏵ 1	2022-02-17 10:02:24	announced

Stella R, Bonadio RS, Cagnin S, Massimino ML, Bertoli A, Peggion C, Perturbations of the Proteome and of Secreted Metabolites in Primary Astrocytes from the hSOD1(G93A) ALS Mouse Model. Int J Mol Sci, 22(13):(2021) [pubmed]

submitter keyword: Spinal cord astrocytes, Amyotrophic lateral sclerosis, Mouse, LC-HRMS/MS

Alessandro Bertoli
contact affiliation	Department of Biomedical Sciences University of Padova via Ugo Bassi, 58b - 35130 - Padova - Italy
contact email	alessandro.bertoli@unipd.it
lab head
Roberto Stella
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie
contact email	rstella@izsvenezie.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026576

PRIDE project URI

• PRIDE
  1. PXD026576
     1. Label: PRIDE project
     2. Name: Perturbations of the proteome in primary astrocytes from the hSOD1(G93A) ALS mouse model