PXD026575

PXD026575 is an original dataset announced via ProteomeXchange.

Title	TAF8 regions important for TFIID lobe B assembly are required for embryonic stem cell survival
Description	The human general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). In eukaryotic cells, TFIID is thought to nucleate RNA polymerase II (Pol II) preinitiation complex formation on all protein coding gene promoters and thus, be crucial for Pol II transcription. TFIID is composed of three lobes, named A, B and C. Structural studies showed that TAF8 is forming a histone fold pair in lobe B and connecting lobe B to lobe C. In the present study, we have investigated the requirement of the different regions of TAF8 for in vitro TFIID assembly, and the importance of certain TAF8 regions for mouse embryonic stem cell (ESC) viability. We have identified a TAF8 region, different from the histone fold domain of TAF8, important for assembling with the 5TAF core complex in lobe B, and four regions of TAF8 each individually required for interacting with TAF2 in lobe C. Moreover, we show that the 5TAF core-interacting TAF8 domain, and the proline rich domain of TAF8 interacting with TAF2, are both required for mouse embryonic stem cell survival. Thus, our study demonstrates that TAF8 regions involving in the connection of lobe B to lobe C are crucial for TFIID function and consequent ESC survival.
HostingRepository	PRIDE
AnnounceDate	2021-10-02
AnnouncementXML	Submission_2021-10-01_17:30:52.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Luo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-08 23:03:29	ID requested
⏵ 1	2021-10-01 17:30:52	announced

Dataset with its publication pending

submitter keyword: TAF8, TFIID, CXMS, embryonic stem cell (ESC)

Jeff Ranish
contact affiliation	Institute of Systems Biology, Seattle, WA.
contact email	jranish@systemsbiology.org
lab head
Jie Luo
contact affiliation	Institute for Systems Biology
contact email	jluo@systemsbiology.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026575
     1. Label: PRIDE project
     2. Name: TAF8 regions important for TFIID lobe B assembly are required for embryonic stem cell survival