PXD026566

PXD026566 is an original dataset announced via ProteomeXchange.

Title	Brain proteome analysis of APPswe mouse model at the early stages of Alzheimer's disease pathology
Description	Plaques consisting of amyloid-β (Aβ) in the brain are characteristic for patients with Alzheimer´s disease. Aβ is enzymatically generated of the amyloid precursor protein (APP). During the disease, Aβ starts to aggregate forming soluble oligomers, soluble protofibrils and eventually insoluble fibrils. Aβ pathology starts mainly at the hippocampus and the surrounding cortical area. Mice overexpressing the A�-precursor protein with the Swedish mutation (APPswe) are one of the most commonly used animal models in Alzheimer’s field. These mice overexpress APP with the Swedish mutation (KM670/671NL), which is adjacent to the β-secretase cleavage site on APP. This results in increased production of Aβ. The aim of this study was to study the proteome of APPswe brain at the early stages (7-8 months old) of the disease, before plaque onset, which starts at around 11-12 months of age in this mouse model. Homogenates of the hippocampus and the rest of the cerebrum from 7-8 months-old APPswe and wild-type (WT) controls were lysed. Afterwards, filter aided tryptic digestion was performed. The resulting peptides were analyzed using LC-UDMSE. The data was searched against a randomized mouse database and label-free quantification analysis was done. In total 2487 proteins were found. In the hippocampus of APPswe mice, the levels of 1283 proteins were significantly altered compared to WT controls. While 1379 proteins were significantly altered in the rest of the cerebrum of APPswe mice compared to WT controls. Among these, mitochondrial proteins and proteins involved in neuron's development were significantly downregulated, suggesting mitochondrial dysfunction and dysregulation of neuron’s growth in the brain of APPswe mice at already this stage of the disease.
HostingRepository	PRIDE
AnnounceDate	2021-06-28
AnnouncementXML	Submission_2021-06-28_06:40:11.197.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026566
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erik Jansson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-08 06:35:10	ID requested
1	2021-06-28 02:30:45	announced
⏵ 2	2021-06-28 06:40:11	announced	2021-06-28: Updated project metadata.

10.1021/ACSCHEMNEURO.1C00303;

Rofo F, Sandbaumh, ü, ter FA, Chourlia A, Metzendorf NG, Morrison JI, Syv, ä, nen S, Andr, é, n PE, Jansson ET, Hultqvist G, Wide-Ranging Effects on the Brain Proteome in a Transgenic Mouse Model of Alzheimer's Disease Following Treatment with a Brain-Targeting Somatostatin Peptide. ACS Chem Neurosci, 12(13):2529-2541(2021) [pubmed]

submitter keyword: tryptic digestion, brain, LC-MS, mice,Alzheimer's disease

Erik Jansson
contact affiliation	Department of Pharmaceutical Biosciences Uppsala University Sweden
contact email	erik.jansson@farmbio.uu.se
lab head
Erik Jansson
contact affiliation	Uppsala University
contact email	erik.jansson@farmbio.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026566

PRIDE project URI

• PRIDE
  1. PXD026566
     1. Label: PRIDE project
     2. Name: Brain proteome analysis of APPswe mouse model at the early stages of Alzheimer's disease pathology