PXD026547

PXD026547 is an original dataset announced via ProteomeXchange.

Title	Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits
Description	In Cleomaceae species, NAD-malic enzyme (NAD-ME) was independently co-opted to participate in C4 photosynthesis. In C4 Cleome species, all NAD-ME genes (NAD-MEα, -ß1 and -ß2) were affected by C4 evolution and are expressed at higher levels than their C3 orthologs in Tarenaya hassleriana. In C3 Cleome, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/ß1 and NAD-MEα/ß2, with similar biochemical properties and tissue occurrence. In the C4 species, Gynandropsis gynandra and Cleome angustifolia, this role is performed only by the NAD-MEα/ß2 heteromer. In these C4 species NAD-MEα/ß1 is preferentially present in leaves of the C4 species where is the predominant isoform. GgNAD-MEα/ß1 exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate. In C4 Cleome NAD-MEα/ß1 represents thus the C4-decarboxylase. GgNAD-MEß1and CaNAD-MEß1 are non-catalytic subunits but impart a stabilizing effect on the associated α-subunit. We conclude that in C4 Cleome the functions of NAD-ME as a TCA cycle associated enzyme and as a C4 photosynthetic decarboxylase coexist in BSC mitochondria and are performed by isoforms originated through associations of differentially adapted subunits.
HostingRepository	PRIDE
AnnounceDate	2021-11-30
AnnouncementXML	Submission_2021-11-30_13:31:43.747.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026547
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Tarenaya hassleriana; NCBI TaxID: 28532; scientific name: Cleome angustifolia; NCBI TaxID: 860688; scientific name: Gynandropsis gynandra; NCBI TaxID: 190802;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-07 07:18:24	ID requested
⏵ 1	2021-11-30 13:31:44	announced

H, ü, dig M, Tronconi MA, Zubimendi JP, Sage TL, Poschmann G, Bickel D, Gohlke H, Maurino VG, Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits. Plant Cell, 34(1):597-615(2022) [pubmed]

submitter keyword: NAD-malic enzyme , C4-photosynthesis, respiration, plant, Cleomaceae, photosynthesis

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine I and Molecular Proteomics Laboratory BMFZ Heinrich Heine University Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026547
     1. Label: PRIDE project
     2. Name: Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits