PXD026514

PXD026514 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CobB interacting proteins identified by SPIDER assay
Description	To assess whether SPIDER could detect transient interactions such as enzymes and their substrates, we examined the interactome of the E. coli protein deacetylase CobB. As the only member of the Sir2 family of deacetylases in E. coli, CobB is known to play a role in many different pathways but their interactors is still incompletely known. Here we applied SPIDER assay and a SILAC-based mass spectrometry strategy to capture and identify CobB Substrates. Biotinylated CobB was incubated with E. coli total lysate labeled with heavy stable isotope. As a control, free biotin was incubated with E. coli total lysate labeled with light stable isotope.
HostingRepository	iProX
AnnounceDate	2021-06-04
AnnouncementXML	Submission_2023-05-06_00:13:14.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuening Wang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-06 22:18:37	ID requested
1	2021-06-06 22:18:59	announced
⏵ 2	2023-05-06 00:13:15	announced	2023-05-06: Update publication information.

Publication List

Jiang HW, Chen H, Zheng YX, Wang XN, Meng Q, Xie J, Zhang J, Zhang C, Xu ZW, Chen ZQ, Wang L, Kong WS, Zhou K, Ma ML, Zhang HN, Guo SJ, Xue JB, Hou JL, Liu ZY, Niu WX, Wang FJ, Wang T, Li W, Wang RN, Dang YJ, Czajkowsky DM, Pei J, Dong JJ, Tao SC, Specific pupylation as IDEntity reporter (SPIDER) for the identification of protein-biomolecule interactions. Sci China Life Sci, 66(8):1869-1887(2023) [pubmed]

Jiang HW, Chen H, Zheng YX, Wang XN, Meng Q, Xie J, Zhang J, Zhang C, Xu ZW, Chen ZQ, Wang L, Kong WS, Zhou K, Ma ML, Zhang HN, Guo SJ, Xue JB, Hou JL, Liu ZY, Niu WX, Wang FJ, Wang T, Li W, Wang RN, Dang YJ, Czajkowsky DM, Pei J, Dong JJ, Tao SC, Specific pupylation as IDEntity reporter (SPIDER) for the identification of protein-biomolecule interactions. Sci China Life Sci, 66(8):1869-1887(2023) [pubmed]

Keyword List

submitter keyword: Escherichia coli proteome, CobB

submitter keyword: Escherichia coli proteome, CobB

Contact List

Shengce Tao
contact affiliation	Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong Universit
contact email	taosc@sjtu.edu.cn
lab head
Xuening Wang
contact affiliation	Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong Universit
contact email	wxnyoyo@sjtu.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI

iProX dataset URI