PXD026503

PXD026503 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteomic Profiling and Identification of Potential Hub Genes in Primary and Recurrent Glioblastoma Multiforme
Description	Glioblastoma multiforme (GBM) is the most lethal brain malignant neoplasm, associated with poor prognosis and high recurrence. The mechanisms involved in GBM development and recurrent remains largely unknown. Hence, it’s considerably imperative to explore the potential targets and unravel the mechanism implicated in GBM tumorigenesis and recurrence. In our study, mass spectrometry-based label-free quantitative proteomics was employed to explore the proteomic profiling in non-paired samples of primary and recurrent GBM. Bioinformatic analysis of differentially expression proteins (DEPs) were applied to screen the hub genes. Subsequently, hub genes expression was verified by western blot and immunohistochemistry (IHC) in GBM tissues, followed by its biological effect on GBM at a cellular level determined. We contoured the divergent landscape of proteome between primary and recurrent GBM, with only ten overlapped DEPs included. The several hub genes screened (e.g., CKAP4 and CANX) and enriched pathways correlated with primary GBM such as post-translation, metabolism, energy pathways and cell growth were profiled. Furthermore, CKAP4 was implicated in proliferation, migration and invasion of A172 and T98G cell lines. However, UQCRC1, together with relative enriched pathways such as oxidative phosphorylation, were screened in recurrent GBM. Establishment of the proteomics and several candidate proteins identified could pose considerable access to understand complex biochemical processes and obtain a better picture of GBM biology.
HostingRepository	iProX
AnnounceDate	2021-06-06
AnnouncementXML	Submission_2023-02-06_19:10:19.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaiyue Xu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-06 21:57:56	ID requested
1	2021-06-06 21:58:15	announced
⏵ 2	2023-02-06 19:10:20	announced	2023-02-07: Update publication information.

Xu K, Zhang K, Ma J, Yang Q, Yang G, Zong T, Wang G, Yan B, Shengxia J, Chen C, Wang L, Wang H, CKAP4-mediated activation of FOXM1 via phosphorylation pathways regulates malignant behavior of glioblastoma cells. Transl Oncol, 29():101628(2023) [pubmed]

submitter keyword: proteomics,primary glioblastoma multiforme,recurrence,CKAP4,UQCRC1

Huijuan Wang
contact affiliation	School of Life Sciences, Northwest University
contact email	whj@nwu.edu.cn
lab head
Kaiyue Xu
contact affiliation	School of Life Sciences, Northwest University, Xi’an, China
contact email	bioxzy163@163.com
dataset submitter

iProX dataset URI