PXD026497

PXD026497 is an original dataset announced via ProteomeXchange.

Title	M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity
Description	Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.
HostingRepository	PRIDE
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2021-07-20_02:37:28.392.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026497
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	shue chen
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-methionine sulfoxide; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-04 09:37:36	ID requested
1	2021-06-06 23:32:04	announced
⏵ 2	2021-07-20 02:37:29	announced	2021-07-20: Updated publication reference for PubMed record(s): 34234130.

Bag I, Chen S, Rosin LF, Chen Y, Liu CY, Yu GY, Lei EP, M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity. Nat Commun, 12(1):4170(2021) [pubmed]

submitter keyword: Drosophila, embryo, nuclear extract, Su(Hw), mass spectrometry

Elissa Lei
contact affiliation	Nuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
contact email	leielissa@niddk.nih.gov
lab head
shue chen
contact affiliation	NIH
contact email	chenshue001@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026497

PRIDE project URI

• PRIDE
  1. PXD026497
     1. Label: PRIDE project
     2. Name: M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity