PXD026495 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | THE INTRA-MITOCHONDRIAL O-GLCNACYLATION SYSTEM ACUTELY REGULATES OXPHOS CAPACITY AND ROS DYNAMICS IN THE HEART |
Description | O-linked N-acetylglucosamination (O-GlcNAcylation) of proteins is increasingly recognized as an important cellular regulatory mechanism. In the heart, nucleocytoplasmic O-GlcNAcylation is involved in the modulation of multiple pathophysiological processes. However, the mechanisms leading to O-GlcNAcylation in mitochondria and the consequences on their function remain poorly understood. In this study, we used an in vitro reconstitution assay to characterize the intra-mitochondrial O-GlcNAc cycling system without potential confounding effects of O-GlcNAcylation in other cellular compartments. We performed a comparative analysis of the O-GlcNAcylome of isolated cardiac mitochondria acutely (30 min) exposed to UDP-GlcNAc in presence/absence of NButGT, a specific O-GlcNAcylation inducer, and evaluated the impact on mitochondrial function. 412 O-GlcNAcylated mitochondrial proteins were identified by mass spectrometry analysis, with 189 (Q<0.05) displaying increased O-GlcNAcylation in response to NButGT. Among the O-GlcNAcylated pathways identified, oxidative phosphorylation was the main affected. These acute changes in protein O-GlcNAcylation were associated with enhanced Complex I (CI) activity, increased maximal respiration in presence of CI substrates, and a striking reduction of mitochondrial ROS release, which could be related to O-GlcNAcylation of subunits within the NADH dehydrogenase module of CI. In conclusion, our work underlines the existence of a dynamic mitochondrial O-GlcNAcylation system capable of rapidly modifying mitochondrial function. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-11 |
AnnouncementXML | Submission_2022-02-11_03:30:54.291.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD026495 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Didier Vertommen |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-04 09:37:36 | ID requested | |
⏵ 1 | 2022-02-11 03:30:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: heart, O-GlcNAc, mitochondria, oxidative phosphorylation |
Contact List
Luc Bertrand |
contact affiliation | Pole of cardiovascular research - Institute of Experimental and Clinical Research (IREC) Université catholique de Louvain Brussels, Belgium |
contact email | luc.bertrand@uclouvain.be |
lab head | |
Didier Vertommen |
contact affiliation | UCL - de Duve Institute, Brussels Belgium |
contact email | didier.vertommen@uclouvain.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026495
- Label: PRIDE project
- Name: THE INTRA-MITOCHONDRIAL O-GLCNACYLATION SYSTEM ACUTELY REGULATES OXPHOS CAPACITY AND ROS DYNAMICS IN THE HEART