PXD026487

PXD026487 is an original dataset announced via ProteomeXchange.

Title	The PXC3 receptor-like kinase constitutively interacts with BAK1 and is involved in vascular development in Arabidopsis.
Description	We designed an experiment to identify BAK1 interactors considering that it could be a coreceptor for the GOLVEN6 (GLV6) signaling peptide involved in lateral root organogenesis in Arabidopsis thaliana. A BAK1pro:BAK1-GFP/bak1-4 line crossed to an estradiol-inducible GLV6 overexpression line (BAK1pro:BAK1-GFP/bak1-4xiGLV6) was treated with mock or estradiol followed by affinity purification mass spectrometry (AP-MS) experiments using anti-GFP beads in microsomal-enriched fractions. Statistical analysis of estradiol-treated vs. mock seedlings did not reveal significant differences. Nevertheless, we remarked that regardless of the treatment, several LRR-RLKs co-eluted with BAK1-GFP, comprising known, as well as, unknown interactors. We confirmed interaction of BAK1 with these LRR-RLKs by BIFC and identified the PXC3 receptor as a BAK1 constitutive interactor, which was further verified by co-IP.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_08:54:32.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana I. Fernandez Salina
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-04 06:01:12	ID requested
⏵ 1	2022-02-17 08:54:33	announced

Xu K, Jourquin J, Njo MF, Nguyen L, Beeckman T, Fernandez AI, . Front Plant Sci, 12():706633(2021) [pubmed]

submitter keyword: Arabidopsis, BAK1, interactors, receptors, LRR-RLK

Tom Beeckman
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium VIB Center for Plant Systems Biology, Ghent, Belgium
contact email	tobee@psb.ugent.be
lab head
Ana I. Fernandez Salina
contact affiliation	VIB-UGent Center for Plant systems Biology
contact email	fernandez.anaibis@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026487

PRIDE project URI

• PRIDE
  1. PXD026487
     1. Label: PRIDE project
     2. Name: The PXC3 receptor-like kinase constitutively interacts with BAK1 and is involved in vascular development in Arabidopsis.