PXD026483

PXD026483 is an original dataset announced via ProteomeXchange.

Title	Purification of human plasma exosomes for proteomics: optimization and application to detect changes in response to exercise
Description	Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli. Interrogation of the protein cargo of EVs is currently limited by the absence of optimal and reproducible approaches for purifying plasma EVs that are suitable for downstream proteomic analyses. We describe a size exclusion chromatography (SEC)-based method to purify EVs from platelet poor plasma (PPP) for proteomics profiling via high-resolution mass spectrometry (SEC-MS). The SEC-MS method identified more proteins with higher precision compared to several conventional EV isolation approaches. We applied the SEC-MS method to identify the unique proteomic signatures of EVs released from platelets, adipocytes, muscle cells, and hepatocytes, with the goal of identifying tissue-specific EV markers. Further, we applied the SEC-MS approach to evaluate the effects of a single bout of exercise on EV proteomic cargo in human plasma.
HostingRepository	PRIDE
AnnounceDate	2021-08-09
AnnouncementXML	Submission_2021-08-09_03:15:58.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Vanderboom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-04 02:52:40	ID requested
⏵ 1	2021-08-09 03:15:59	announced

Vanderboom PM, Dasari S, Ruegsegger GN, Pataky MW, Lucien F, Heppelmann CJ, Lanza IR, Nair KS, A size-exclusion-based approach for purifying extracellular vesicles from human plasma. Cell Rep Methods, 1(3):(2021) [pubmed]

submitter keyword: size-exclusion chromatography, ultracentrifugation, mass spectrometry, bioinformatics, high intensity exercise

K Sreekumaran Nair
contact affiliation	Endocrine Research Unit, Mayo Clinic, Rochester MN
contact email	Nair@mayo.edu
lab head
Patrick Vanderboom
contact affiliation	Mayo Clinic
contact email	vanderboom.patrick@mayo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026483

PRIDE project URI

• PRIDE
  1. PXD026483
     1. Label: PRIDE project
     2. Name: Purification of human plasma exosomes for proteomics: optimization and application to detect changes in response to exercise