PXD026483 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Purification of human plasma exosomes for proteomics: optimization and application to detect changes in response to exercise |
Description | Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli. Interrogation of the protein cargo of EVs is currently limited by the absence of optimal and reproducible approaches for purifying plasma EVs that are suitable for downstream proteomic analyses. We describe a size exclusion chromatography (SEC)-based method to purify EVs from platelet poor plasma (PPP) for proteomics profiling via high-resolution mass spectrometry (SEC-MS). The SEC-MS method identified more proteins with higher precision compared to several conventional EV isolation approaches. We applied the SEC-MS method to identify the unique proteomic signatures of EVs released from platelets, adipocytes, muscle cells, and hepatocytes, with the goal of identifying tissue-specific EV markers. Further, we applied the SEC-MS approach to evaluate the effects of a single bout of exercise on EV proteomic cargo in human plasma. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-09 |
AnnouncementXML | Submission_2021-08-09_03:15:58.742.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Patrick Vanderboom |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-04 02:52:40 | ID requested | |
⏵ 1 | 2021-08-09 03:15:59 | announced | |
Publication List
Vanderboom PM, Dasari S, Ruegsegger GN, Pataky MW, Lucien F, Heppelmann CJ, Lanza IR, Nair KS, A size-exclusion-based approach for purifying extracellular vesicles from human plasma. Cell Rep Methods, 1(3):(2021) [pubmed] |
Keyword List
submitter keyword: size-exclusion chromatography, ultracentrifugation, mass spectrometry, bioinformatics, high intensity exercise |
Contact List
K Sreekumaran Nair |
contact affiliation | Endocrine Research Unit, Mayo Clinic, Rochester MN |
contact email | Nair@mayo.edu |
lab head | |
Patrick Vanderboom |
contact affiliation | Mayo Clinic |
contact email | vanderboom.patrick@mayo.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026483 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026483
- Label: PRIDE project
- Name: Purification of human plasma exosomes for proteomics: optimization and application to detect changes in response to exercise