PXD026474

PXD026474 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteomic analysis of activated P. falciparum gametocytes, wild type NF54 and PfCDPK4 knockout
Description	Gametocytes of the malaria parasite Plasmodium are taken up by the mosquito vector with an infectious blood meal, representing a critical stage for parasite transmission. It is well known that calcium dependent protein kinases (CDPKs) play key roles in calcium-mediated signaling across the complex life cycle of the parasite and we sought to understand their role in transmission from the mammalian host to the mosquito vector. Here, we investigated the role of CDPK4 in the life cycle of the human-infective parasite Plasmodium falciparum . We created Plasmodium falciparum cdpk4 knockout parasites by targeted gene deletion, and this deletion had no effect on blood stage development or gametocyte development. However, cdpk4 knockout parasites showed a severe defect in male gametogenesis and the emergence of male flagellated gametes. To understand this defect, we performed mass spectrometry-based phosphoproteomic analysis of wild type and Plasmodium falciparum cdpk4 knockout late gametocyte stages in order to identify key CDPK4-mediated phosphorylation events that may be important for the regulation of male gametogenesis.
HostingRepository	MassIVE
AnnounceDate	2021-06-05
AnnouncementXML	Submission_2021-06-05_13:17:42.098.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristian Swearingen
SpeciesList	scientific name: Plasmodium falciparum NF54; NCBI TaxID: 5843;
ModificationList	O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-03 16:26:44	ID requested
⏵ 1	2021-06-05 13:17:42	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Plasmodium, P. falciparum, gametocyte, phosphorylation, CDPK4

submitter keyword: Plasmodium, P. falciparum, gametocyte, phosphorylation, CDPK4

Contact List

Kristian Swearingen
contact affiliation	Institute for Systems Biology
contact email	kristian.swearingen@isbscience.org
lab head
Kristian Swearingen
contact affiliation	Institute for Systems Biology
contact email	kristian.swearingen@isbscience.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087569/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087569/