Updated FTP location. Parental and 5-fluorouracil (5-FU)-resistant HCT15 cells were treated with 5-FU or vehicle for 12h and assembled into an IMPRINTS-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC, 54ºC, and 57ºC) with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on the mass spectrometer. This entry contains two datasets: 1. 5-FU in parental vs. resistant HCT15 (file names “12h 5FU_par vs resHCT15”) for data referred to as HCT15_Par_5FU and HCT15_Res_5FU in the associated publication. The labelling order of samples in the TMT10 channels for this dataset was as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_Parental_Vehicle (126), Biorep1_Parental_5FU_100µM (127N), Biorep1_Resistant_5FU_16µM (127C), Biorep2_ Parental_Vehicle (128N), Biorep2_ Parental_5FU_100µM (128C), Biorep2_ Resistant_5FU_16µM (129N), Biorep3_ Parental_Vehicle (129C), Biorep3_ Parental_5FU_100µM (130N), Biorep3_ Resistant_5FU_16µM (130C), mixed_control (131). 2. 5-FU in resistant HCT15 (file names “12h 5FU_ResHCT15_diff”) for data referred to as HCT15_Res_Diff in the in the associated publication. The labelling order of samples in the TMT10 channels for this dataset was as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_ Resistant_5FU_16µM (128N), Biorep2_ Resistant_5FU_16µM (128C), Biorep3_ Resistant_5FU_16µM (129N), Biorep1_ Resistant_5FU_100µM (129C), Biorep2_ Resistant_5FU_100µM (130N), Biorep3_ Resistant_5FU_100µM (130C), mixed_control (131). For additional details see the methods section of the associated publication.