PXD026471

PXD026471 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	IMPRINTS-CETSA, FUDR and FUR in MCF7 cells, 2h and 12h incubation
Description	MCF7 cells were treated with floxuridine (FUDR), fluorouridine (FUR), or vehicle and assembled into an IMPRINTS-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC, 54ºC, and 57ºC) with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on the mass spectrometer. This entry contains two IMPRINTS-CETSA datasets: 1. MCF7 incubated with Vehicle/FUDR/FUR for 2 hours 2. MCF7 incubated with Vehicle/FUDR/FUR for 12 hours The labelling order of samples in the TMT10 channels for both datasets was as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_Vehicle (126), Biorep1_FUDR (127N), Biorep1_FUR (127C), Biorep2_Vehicle (128N), Biorep2_FUDR (128C), Biorep2_FUR (129N), Biorep3_Vehicle (129C), Biorep3_FUDR (130N), Biorep3_FUR (130C), mixed_control (131). For additional details see the methods section of the associated publication.
HostingRepository	jPOST
AnnounceDate	2021-07-15
AnnouncementXML	Submission_2022-09-18_02:25:27.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Smaranda Bacanu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; unknown modification; unknown modification
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-03 12:59:31	ID requested
1	2021-07-15 05:57:54	announced
2	2021-08-25 10:00:12	announced	2021-08-26: Updated PubMed.
⏵ 3	2022-09-18 02:25:28	announced	2022-09-18: Updated FTP location.

Publication List

Liang YY, Bacanu S, Sreekumar L, Ramos AD, Dai L, Michaelis M, Cinatl J, Seki T, Cao Y, Coffill CR, Lane DP, Prabhu N, Nordlund P, CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity. Cell Chem Biol, 29(4):572-585.e8(2022) [pubmed]

Liang YY, Bacanu S, Sreekumar L, Ramos AD, Dai L, Michaelis M, Cinatl J, Seki T, Cao Y, Coffill CR, Lane DP, Prabhu N, Nordlund P, CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity. Cell Chem Biol, 29(4):572-585.e8(2022) [pubmed]

Keyword List

submitter keyword: MS-CETSA, IMPRINTS-CETSA, FUDR, FUR, 5-fluorouracil metabolites

submitter keyword: MS-CETSA, IMPRINTS-CETSA, FUDR, FUR, 5-fluorouracil metabolites

Contact List

Pär Nordlund
lab head
Smaranda Bacanu
contact affiliation	Karolinska Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001190/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001190/