Updated FTP location. MCF7 xenograft-bearing mice were treated intravenously for 22h with either vehicle or different 5-FU doses (10, 50, 100, and 150 mg/kg). Each xenograft was cut in similar-sized pieces and assembled into an ITDRCETSA scheme with 2 different heating temperatures (37ºC, 52ºC) and 3 xenograft pieces for each temperature. The ITDRCETSA samples were labelled using TMT10-plex. The labelling order of samples in the TMT10 channels for was as follows: Tumour pieces 1, 2, and 3: Vehicle_52C (126), 10mg/kg_52C (127N), 50mg/kg_52C (127C), 100mg/kg_52C (128N), 150mg/kg_52C (128C), Vehicle_37C (129N), 10mg/kg_37C (129C), 50mg/kg_37C (130N), 100mg/kg_37C (130C), 150mg/kg_37C (131). For additional details see the methods section of the associated publication.