PXD026462

PXD026462 is an original dataset announced via ProteomeXchange.

Title	Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase
Description	We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellent compatibility with primary human macrophages, and several cell lines, without cytotoxic effect. However, stationary-phase MVs showed slightly higher uptake into primary macrophages. Both vesicle types enhanced the release of inflammatory cytokines tumor necrosis factor and interleukin-6 from primary human macrophages. Proteomic analysis revealed similarities in vesicular immunogenic proteins in both vesicle types, such as pneumolysin, pneumococcal surface protein A, IgA1 protease, but stationary-phase MVs showed significantly lower autolysin levels, than death-phase MVs.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_03:59:28.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Kroniger
SpeciesList	scientific name: Streptococcus pneumoniae R6; NCBI TaxID: 171101;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-03 06:04:55	ID requested
⏵ 1	2022-02-16 03:59:29	announced

Mehanny M, Kroniger T, Koch M, Hoppst, ä, dter J, Becher D, Kiemer AK, Lehr CM, Fuhrmann G, Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase. Adv Healthc Mater, 11(5):e2101151(2022) [pubmed]

submitter keyword: Bacterial membrane vesicles, death-phase, stationary-phase, immunogenicity, proteomics, vaccine, primary macrophages

Dörte Becher
contact affiliation	Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Tobias Kroniger
contact affiliation	Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald
contact email	kronigert@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026462

PRIDE project URI

• PRIDE
  1. PXD026462
     1. Label: PRIDE project
     2. Name: Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase