PXD026437

PXD026437 is an original dataset announced via ProteomeXchange.

Title	Explore the lactation function of Protein phosphorylation sites in goat mammary tissues by phosphoproteome
Description	Background: Protein phosphorylation plays an important role in lactation. Differentially modified phosphorylation sites between peak lactation (PL, 90 days postpartum) and late lactation (LL, 280 days postpartum) were investigated using an integrated approach, namely, liquid chromatography with tandem mass spectrometry (LC-MS/MS) and tandem mass tag (TMT) labelling, to know the molecular changes in different stages of goat mammary tissues. Results: A total of 1,938 (1,111 up-regulated, 827 down-regulated) differentially modified phosphorylation sites of 1,172 proteins were identified (P values < 0.05 and fold change of phosphorylation ratios > 1.5). In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that calcium signalling pathway, oxytocin signalling pathway and MAPK signalling pathway were enriched. The results of western blot showed phosphorylation levels of ACACA, EIF4EBP1 and IRS1 increased and JUN decreased in PL compared with LL. The results were consistent with the results of phosphoproteome. Conclusions: In this study, we first differentially proteins modified phosphorylation sites between PL and LL in goat mammary tissues. On the other hand, these results analysis indicate that multiple differentially modified phosphorylation sites of FASN, ACACA, mTOR, PRKAA, IRS1, RPS6KB, EIF4EBP1, TSC2 and proteins of Calcium signalling pathway, Oxytocin signalling pathway, and MAPK signalling pathway are worthy of further exploration.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_08:54:29.472.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaopeng An
SpeciesList	scientific name: Capra hircus; NCBI TaxID: 9925;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-02 15:27:56	ID requested
⏵ 1	2022-02-17 08:54:31	announced

Zhu C, Zhu J, Duan Q, Jiang Y, Yin H, He Y, Li F, An XP, Exploration of the lactation function of protein phosphorylation sites in goat mammary tissues by phosphoproteome analysis. BMC Genomics, 22(1):703(2021) [pubmed]

submitter keyword: Phosphoproteome

lactation

IRS1

β-casein

triglycerides

goat

Xiaopeng An
contact affiliation	College of Animal Science and Technology, Northwest A&F University, Yangling
contact email	anxiaopengdky@163.com
lab head
Xiaopeng An
contact affiliation	Northwest A&F University
contact email	anxiaopengdky@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026437
     1. Label: PRIDE project
     2. Name: Explore the lactation function of Protein phosphorylation sites in goat mammary tissues by phosphoproteome