PXD026412 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FIRST DETECTION OF SARS-COV-2 PROTEINS IN WASTEWATER SAMPLES BY MASS SPECTROMETRY |
Description | On March 12, 2020, the World Health Organization (WHO) declared COVID-19 as a global pandemic. COVID-19 is produced by a novel β-coronavirus known as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) [1]. Several studies have detected SARS-CoV-2 RNA in urine, feces, and other biofluids from both symptomatic and asymptomatic people with COVID-19 [2], suggesting that SARS-CoV-2 RNA could be detected in human wastewater [3]. Thus, wastewater-based epidemiology (WBE) is now used as an approach to monitor COVID-19 prevalence in many different places around the world [4-10] . Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the most common SARS-CoV-2 detection method in WBE, but there are other methods for viral biomolecule detection that could work as well. The aim of this study was to evaluate the presence of SARS-CoV-2 proteins in untreated wastewater (WW) influents collected from six wastewater treatment plants (WWTPs), from Durham Region, Ontario, Canada, using a LC-MS/MS-based proteomics approach. We identified many SARS-CoV-2 proteins in these wastewater samples, with peptides from pp1ab being the most consistently detected and with consistent abundance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:35:37.145.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Denina Simmons |
SpeciesList | scientific name: Coronavirus PREDICT CoV-2; NCBI TaxID: 2042546; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-01 22:10:09 | ID requested | |
1 | 2024-10-08 11:09:14 | announced | |
⏵ 2 | 2024-10-22 05:35:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: SARS-CoV-2, proteomics, liquid-chromatography mass spectrometry, wastewater, COVID-19 |
Contact List
Denina Simmons |
contact affiliation | Aquatic Omics Lab, Science department, Ontario Tech University |
contact email | Denina.Simmons@ontariotechu.ca |
lab head | |
Denina Simmons |
contact affiliation | Ontario Tech University |
contact email | denina.simmons@uoit.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026412
- Label: PRIDE project
- Name: FIRST DETECTION OF SARS-COV-2 PROTEINS IN WASTEWATER SAMPLES BY MASS SPECTROMETRY