PXD026408

PXD026408 is an original dataset announced via ProteomeXchange.

Title	RADIATION-INDUCED MODIFICATIONS IN MICRO-RNA AND PROTEOMIC CARGO IN PLASMA-DERIVED EXTRACELLULAR VESICLES
Description	In this study, to delineate the radiation-induced changes in miRNAs and protein cargo, we established the microRNA and proteome profiles of plasma EVs collected from mice that had been sham- (SI), whole body (WBI), or partial body irradiated (PBI) with 2 Gy X-rays 24 hours before. The radiation-induced changes in the miRNA and protein composition of EVs can influence the stress response of distant cells by propagating damaging or protective signals. Testing EVs in functional assays may be a read out of the multiple radiation-induced signaling networks, crucial for gaining an understanding of the EVs properties. Therefore, to functionally elucidate the pathophysiological changes in the contents of EVs induced by irradiation, we here investigated the EV-induced cellular responses in vivo, by injecting WBI, PBI and SI plasma-derived EVs in the cerebellum of neonatal mice, highly susceptible to radiation injury, and evaluating the level of apoptosis.
HostingRepository	PRIDE
AnnounceDate	2022-03-10
AnnouncementXML	Submission_2022-03-10_01:45:33.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine von Toerne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-01 21:59:13	ID requested
⏵ 1	2022-03-10 01:45:33	announced

Dataset with its publication pending

submitter keyword: MiRNome, proteomics, radiation

ionizing radiation

neonatal cerebellum

apoptosis

Maria Teresa Mancuso
contact affiliation	Laboratory of Biomedical Technologies, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy
contact email	mariateresa.mancuso@enea.it
lab head
Christine von Toerne
contact affiliation	Helmholtz Zentrum München
contact email	vontoerne@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD026408

PRIDE project URI

• PRIDE
  1. PXD026408
     1. Label: PRIDE project
     2. Name: RADIATION-INDUCED MODIFICATIONS IN MICRO-RNA AND PROTEOMIC CARGO IN PLASMA-DERIVED EXTRACELLULAR VESICLES