PXD026400

PXD026400 is an original dataset announced via ProteomeXchange.

Title	Conserved core effectors determine multi-host plant compatibility in a vascular wilt fungus
Description	Fungal interactions with plant roots, either beneficial or detrimental, have a major impact on agriculture and ecosystems1. The soil inhabiting ascomycete Fusarium oxysporum (Fo) constitutes a species complex of worldwide distribution causing vascular wilt in more than a hundred different crops2,3. Individual isolates of the fungus exhibit host-specific pathogenicity, determined by proteinaceous effectors termed secreted in xylem (SIX)4,5. However, such isolates can also colonize roots of non-host plants asymptomatically as endophytes, or even protect the plant against pathogenic isolates6,7. The molecular determinants of multi-host plant colonization are currently unknown. Here, we identified a set of fungal effectors termed ERCs (Early Root Compatibility effectors), which are secreted during early biotrophic growth of Fo on both host and non-host plants. In contrast to SIX effectors, which are encoded on lineage specific (LS) genomic regions5,8, ERCs are encoded on core genomic regions and broadly conserved across the Fo species complex. Targeted deletion of ERC genes in pathogenic Fo isolate resulted in reduced virulence on the host plant and rapid activation of plant immune responses, while in a non-pathogenic isolate it led to impaired root colonization and loss of biocontrol ability. Strikingly, some ERCs also contribute to Fo infection on the non-vascular land plant Marchantia polymorpha. Our results reveal an evolutionarily conserved mechanism for multi-host colonization by root infecting fungi.
HostingRepository	PRIDE
AnnounceDate	2022-09-12
AnnouncementXML	Submission_2022-09-12_09:28:26.793.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026400
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Amey Redkar
SpeciesList	scientific name: Fusarium oxysporum f. sp. lycopersici 4287; NCBI TaxID: 426428;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-01 15:58:25	ID requested
⏵ 1	2022-09-12 09:28:27	announced

Redkar A, Sabale M, Schudoma C, Zechmann B, Gupta YK, L, ó, pez-Berges MS, Venturini G, Gimenez-Ibanez S, Turr, à D, Solano R, Di Pietro A, Conserved secreted effectors contribute to endophytic growth and multihost plant compatibility in a vascular wilt fungus. Plant Cell, 34(9):3214-3232(2022) [pubmed]

submitter keyword: Fusarium oxysporum, Apoplastic fluid, LC-MSMS

Antonio Di Pietro
contact affiliation	Department of Genetics, Universidad de Cordoba, Spain
contact email	ge2dipia@uco.es
lab head
Amey Redkar
contact affiliation	Universidad de Cordoba, Spain
contact email	ameyred@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD026400

PRIDE project URI

• PRIDE
  1. PXD026400
     1. Label: PRIDE project
     2. Name: Conserved core effectors determine multi-host plant compatibility in a vascular wilt fungus