Updated publication reference for PubMed record(s): 34962809. The aim of this study was to develop a Peptide Atlas repository for Labeo rohita. Using LC-MS/MS, we have performed in-depth proteomics analysis of 19 different sample types, including 17 tissue samples, plasma from female fish and embryo 4-day post fertilization. Whole analysis resulted in the identification of more than 6015 confident canonical proteins along with ~3000 non canonical peoteins corresponding to more than 150 thousands peptides. This proteomic dataset would complement the recently published genome to accelerate further research.